PDBe 4v1x

X-ray diffraction
2.2Å resolution

The structure of the hexameric atrazine chlorohydrolase, AtzA

Released:
Source organism: Pseudomonas sp. ADP
Primary publication:
The structure of the hexameric atrazine chlorohydrolase AtzA.
OpenAccess logo Acta Crystallogr. D Biol. Crystallogr. 71 710-20 (2015)
PMID: 25760618

Function and Biology Details

Reaction catalysed:
Atrazine + H(2)O = 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Atrazine chlorohydrolase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 494 amino acids
Theoretical weight: 54.72 KDa
Source organism: Pseudomonas sp. ADP
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P72156 (Residues: 1-474; Coverage: 100%)
Gene name: atzA
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 117.269Å b: 146.056Å c: 196.361Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.196
Expression system: Escherichia coli BL21(DE3)