PDBe 4v1c

X-ray diffraction
2.95Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
NAD-dependent protein deacetylase sirtuin-3, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 30.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NTG7 (Residues: 121-395; Coverage: 69%)
Gene names: SIR2L3, SIRT3
Sequence domains: Sir2 family
Structure domains:
NAD-dependent protein deacetylase sirtuin-3, mitochondrial Chains: B, E, G, K
Molecule details ›
Chains: B, E, G, K
Length: 275 amino acids
Theoretical weight: 30.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NTG7 (Residues: 121-395; Coverage: 69%)
Gene names: SIR2L3, SIRT3
Sequence domains: Sir2 family
Structure domains:
PEPTIDE Chains: C, D, F, H, J, L
Molecule details ›
Chains: C, D, F, H, J, L
Length: 7 amino acids
Theoretical weight: 932 Da
Source organism: synthetic construct
NAD-dependent protein deacetylase sirtuin-3, mitochondrial Chain: I
Molecule details ›
Chain: I
Length: 275 amino acids
Theoretical weight: 30.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NTG7 (Residues: 121-395; Coverage: 69%)
Gene names: SIR2L3, SIRT3
Sequence domains: Sir2 family
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 138.085Å b: 138.085Å c: 225.342Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.224 0.278
Expression system: Escherichia coli BL21(DE3)