› 4uw0

X-ray diffraction
3.87Å resolution

Low resolution structure of WbdD with C-terminal bundle ordered to residue 505

Released:
DOI: 10.2210/pdb4uw0/pdb
PDB entry 4uw0 coloured by chain, front view.
PDB entry 4uw0 coloured by chain, side view.
PDB entry 4uw0 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide.
Hagelueken G, Clarke BR, Huang H, Tuukkanen A, Danciu I, Svergun DI, Hussain R, Liu H, Whitfield C, Naismith JH
Nat Struct Mol Biol 22 50-56 (2015)
PMID: 25504321

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WBDD Chain: A
Molecule details ›
Chain: A
Length: 505 amino acids
Theoretical weight: 57.75 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand SAM 1 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: I23
Unit cell:
a: 181.29Å b: 181.29Å c: 181.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.268 0.341
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Circular dichroism spectroscopy of membrane proteins.
Miles et al. (2016)
5 more

2 mentions without citation

Current Progress in the Structural and Biochemical Characterization of Proteins Involved in the Assembly of Lipopolysaccharide.
Bohl et al. (2018)
1 more