PDBe 4uow

X-ray diffraction
3.3Å resolution

Crystal structure of the titin M10-Obscurin Ig domain 1 complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Obscurin Chains: 0, 2, 4, 6, 8, A, C, E, G, I, K, M, O, Q, S, U, W, Y
Molecule details ›
Chains: 0, 2, 4, 6, 8, A, C, E, G, I, K, M, O, Q, S, U, W, Y
Length: 97 amino acids
Theoretical weight: 10.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5VST9 (Residues: 110-203; Coverage: 1%)
Gene names: KIAA1556, KIAA1639, OBSCN
Sequence domains: Immunoglobulin I-set domain
Structure domains: Immunoglobulins
Titin Chains: 1, 3, 5, 7, 9, B, D, F, H, J, L, N, P, R, T, V, X, Z
Molecule details ›
Chains: 1, 3, 5, 7, 9, B, D, F, H, J, L, N, P, R, T, V, X, Z
Length: 93 amino acids
Theoretical weight: 9.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8WZ42 (Residues: 34058-34150; Coverage: 0%)
  • Best match: Q8WZ42-6 (Residues: 5380-5462)
Gene name: TTN
Sequence domains: Immunoglobulin I-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P3221
Unit cell:
a: 201.56Å b: 201.56Å c: 183.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.177 0.21
Expression system: Escherichia coli BL21(DE3)