› 4uoe

X-ray diffraction
2.05Å resolution

Crystal Structure of Plasmodium Falciparum Spermidine Synthase in Complex with 5'-Deoxy-5'-Methylioadenosine and 4-Aminomethylaniline

Released:
DOI: 10.2210/pdb4uoe/pdb
PDB entry 4uoe coloured by chain, front view.
PDB entry 4uoe coloured by chain, side view.
PDB entry 4uoe coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
Three-dimensional structures of Plasmodium falciparum spermidine synthase with bound inhibitors suggest new strategies for drug design.
Sprenger J, Svensson B, Hålander J, Carey J, Persson L, Al-Karadaghi S
Acta Crystallogr D Biol Crystallogr 71 484-93 (2015)
PMID: 25760598

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PABS domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 281 amino acids
Theoretical weight: 32.08 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8II73 (Residues: 41-321; Coverage: 88%)
Gene name: PF3D7_1129000
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MTA 3 x MTA
Ligand 4ZY 3 x 4ZY
Ligand GOL 2 x GOL
Ligand 1PG 1 x 1PG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: C2
Unit cell:
a: 198.05Å b: 134.616Å c: 48.306Å
α: 90° β: 95.33° γ: 90°
R-values:
R R work R free
0.201 0.199 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Plasmodium AdoMetDC/ODC bifunctional enzyme is essential for male sexual stage development and mosquito transmission.
Hart et al. (2016)
5 more

3 mentions without citation

Polyamines in protozoan pathogens.
Phillips MA. (2018)
2 more