PDBe 4un2

X-ray diffraction
1.51Å resolution

Crystal structure of the UBA domain of Dsk2 in complex with Ubiquitin

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Ubiquitin domain-containing protein DSK2 Chain: B
Molecule details ›
Chain: B
Length: 48 amino acids
Theoretical weight: 5.34 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48510 (Residues: 328-373; Coverage: 12%)
Gene names: DSK2, SHE4, YM8021.02, YMR276W
Structure domains: DNA helicase RuvA subunit, C-terminal domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 77.971Å b: 77.972Å c: 49.485Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.157 0.196
Expression system: Escherichia coli BL21(DE3)