PDBe 4uis

Electron Microscopy
4.4Å resolution

The cryoEM structure of human gamma-Secretase complex

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis of human γ-secretase assembly.
OpenAccess logo Proc. Natl. Acad. Sci. U.S.A. 112 6003-8 (2015)
PMID: 25918421
Related structures: EMD-2974

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Nicastrin Chain: A
Molecule details ›
Chain: A
Length: 588 amino acids
Theoretical weight: 63.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92542 (Residues: 42-665; Coverage: 84%)
Gene names: KIAA0253, NCSTN, UNQ1874/PRO4317
Sequence domains: Nicastrin small lobe
Presenilin-1 CTF12 Chain: B
Molecule details ›
Chain: B
Length: 241 amino acids
Theoretical weight: 23.55 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49768 (Residues: 404-463; Coverage: 13%)
  • Best match: P49768-4 (Residues: 81-99, 100-100)
Gene names: AD3, PS1, PSEN1, PSNL1
GAMMA-SECRETASE Chain: C
Molecule details ›
Chain: C
Length: 196 amino acids
Theoretical weight: 16.7 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
GAMMA-SECRETASE Chain: D
Molecule details ›
Chain: D
Length: 62 amino acids
Theoretical weight: 5.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Endolysin Chain: G
Molecule details ›
Chain: G
Length: 162 amino acids
Theoretical weight: 18.44 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: D9IEF7 (Residues: 1-162; Coverage: 99%)
Gene names: T4Tp126, e
Sequence domains: Phage lysozyme

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-2974
Expression system: Homo sapiens