PDBe 4uf6

X-ray diffraction
3.69Å resolution

UCH-L5 in complex with ubiquitin-propargyl bound to an activating fragment of INO80G

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin carboxyl-terminal hydrolase isozyme L5 Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 331 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5K5 (Residues: 1-329; Coverage: 100%)
  • Best match: Q9Y5K5-3 (Residues: 1-328)
Gene names: AD-019, CGI-70, UCH37, UCHL5
Sequence domains:
Ubiquitin Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 76 amino acids
Theoretical weight: 8.56 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P0CG47 (Residues: 153-227; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
Nuclear factor related to kappa-B-binding protein Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 65 amino acids
Theoretical weight: 7.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6P4R8 (Residues: 40-101; Coverage: 5%)
Gene names: INO80G, NFRKB

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 152.084Å b: 137.788Å c: 98.916Å
α: 90° β: 102.54° γ: 90°
R-values:
R R work R free
0.24 0.238 0.269
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided