PDBe 4uel

X-ray diffraction
2.3Å resolution

UCH-L5 in complex with ubiquitin-propargyl bound to the RPN13 DEUBAD domain

Released:

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin carboxyl-terminal hydrolase isozyme L5 Chain: A
Molecule details ›
Chain: A
Length: 331 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5K5 (Residues: 1-329; Coverage: 100%)
  • Best match: Q9Y5K5-3 (Residues: 1-328)
Gene names: AD-019, CGI-70, UCH37, UCHL5
Sequence domains:
Structure domains:
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.56 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family
Proteasomal ubiquitin receptor ADRM1 Chain: C
Molecule details ›
Chain: C
Length: 127 amino acids
Theoretical weight: 13.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16186 (Residues: 266-388; Coverage: 30%)
Gene names: ADRM1, GP110
Sequence domains: UCH-binding domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 59.34Å b: 98.6Å c: 100.211Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.234
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided