PDBe 4u32

X-ray diffraction
1.65Å resolution

Human mesotrypsin complexed with HAI-2 Kunitz domain 1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kunitz-type protease inhibitor 2 Chain: X
Molecule details ›
Chain: X
Length: 55 amino acids
Theoretical weight: 6.3 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O43291 (Residues: 34-88; Coverage: 24%)
Gene names: HAI2, KOP, SPINT2
Structure domains: Pancreatic trypsin inhibitor Kunitz domain
Trypsin-3 Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 24.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35030 (Residues: 81-304; Coverage: 74%)
Gene names: PRSS3, PRSS4, TRY3, TRY4
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P212121
Unit cell:
a: 37.194Å b: 70.19Å c: 123.033Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.213
Expression systems:
  • Komagataella pastoris
  • Escherichia coli