PDBe 4u30

X-ray diffraction
2.5Å resolution

Human mesotrypsin complexed with bikunin Kunitz domain 2


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Trypsin-3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 224 amino acids
Theoretical weight: 24.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P35030 (Residues: 81-304; Coverage: 74%)
Gene names: PRSS3, PRSS4, TRY3, TRY4
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Trypstatin Chains: W, X, Y, Z
Molecule details ›
Chains: W, X, Y, Z
Length: 59 amino acids
Theoretical weight: 6.45 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
  • Canonical: P02760 (Residues: 283-287, 288-340; Coverage: 17%)
Gene names: AMBP, HCP, ITIL
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P3
Unit cell:
a: 164Å b: 164Å c: 81.021Å
α: 90° β: 90° γ: 120°
R R work R free
0.19 0.188 0.223
Expression systems:
  • Escherichia coli
  • Komagataella pastoris