PDBe 4tks

X-ray diffraction
3.2Å resolution

Native-SAD phasing for human EGFR kinase domain.

Released:
Source organism: Homo sapiens
Primary publication:
Multi-crystal native SAD analysis at 6 keV.
Acta Crystallogr. D Biol. Crystallogr. 70 2544-57 (2014)
PMID: 25286840

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epidermal growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 37.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: NEW P00533 (Residues: 695-1022; Coverage: 28%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine kinase
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I23
Unit cell:
a: 146.161Å b: 146.161Å c: 146.161Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.209
Expression system: Spodoptera frugiperda