PDBe 4rqs

X-ray diffraction
4.49Å resolution

Crystal structure of fully glycosylated HIV-1 gp120 core bound to CD4 and 17b Fab

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
T-cell surface glycoprotein CD4 Chain: B
Molecule details ›
Chain: B
Length: 185 amino acids
Theoretical weight: 20.5 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: NEW P01730 (Residues: 26-208; Coverage: 42%)
Gene name: CD4
Sequence domains: Immunoglobulin C2-set domain
17b Fab Light Chain Chain: C
Molecule details ›
Chain: C
Length: 214 amino acids
Theoretical weight: 23.4 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
17b Fab Heavy Chain Chain: D
Molecule details ›
Chain: D
Length: 229 amino acids
Theoretical weight: 24.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Surface protein gp120 Chain: G
Molecule details ›
Chain: G
Length: 313 amino acids
Theoretical weight: 34.84 KDa
Source organism: Human immunodeficiency virus type 1 (isolate YU2)
Expression system: Homo sapiens
UniProt:
  • Canonical: NEW P35961 (Residues: 82-126, 191-293, 325-479; Coverage: 37%)
Gene name: env

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 171.318Å b: 171.318Å c: 151.857Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.278 0.276 0.322
Expression systems:
  • Cricetulus griseus
  • Homo sapiens