› 4rgp

X-ray diffraction
2.3Å resolution

Crystal Structure of Uncharacterized CRISPR/Cas System-associated Protein Csm6 from Streptococcus mutans

Released:
DOI: 10.2210/pdb4rgp/pdb
PDB entry 4rgp coloured by chain, front view.
PDB entry 4rgp coloured by chain, side view.
PDB entry 4rgp coloured by chain, top view.
Source organism: Streptococcus mutans UA159
Entry authors: Kim Y, Li H, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine protein kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 30.67 KDa
Source organism: Streptococcus mutans UA159
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DTU7 (Residues: 2-255; Coverage: 100%)
Gene name: SMU_1226c
Sequence domains: CRISPR-associated protein (Cas_Csm6)

Ligands and Environments

3 bound ligands:
Ligand CA 7 x CA
Ligand PGE 1 x PGE
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 75.511Å b: 78.238Å c: 92.269Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.196 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links