PDBe 4rf0

X-ray diffraction
2.8Å resolution

Crystal structure of the Middle-East respiratory syndrome coronavirus papain-like protease in complex with ubiquitin (space group P6522)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ORF1ab protein Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 36.05 KDa
Source organism: Human betacoronavirus 2c Jordan-N3/2012
Expression system: Escherichia coli
UniProt:
  • Canonical: M4STU1 (Residues: 1480-1803; Coverage: 5%)
Gene name: ORF1ab
Structure domains:
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 75 amino acids
Theoretical weight: 8.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62987 (Residues: 1-75; Coverage: 59%)
Gene names: UBA52, UBCEP2
Sequence domains: Ubiquitin family

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P6522
Unit cell:
a: 176.972Å b: 176.972Å c: 84.549Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.24 0.281
Expression system: Escherichia coli