PDBe 4r5i

X-ray diffraction
1.97Å resolution

Crystal structure of the DnaK C-terminus with the substrate peptide NRLLLTG

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein DnaK Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 25.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6Y8 (Residues: 389-607; Coverage: 34%)
Gene names: JW0013, b0014, dnaK, groP, grpF, seg
Structure domains:
HSP70/DnaK Substrate Peptide: NRLLLTG Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 787 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I222
Unit cell:
a: 48.243Å b: 57.911Å c: 180.076Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.218
Expression systems:
  • Escherichia coli
  • Not provided