PDBe 4qo1

X-ray diffraction
1.92Å resolution

p53 DNA binding domain in complex with Nb139

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nb139 Nanobody against the DNA-binding domain of p53 Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 16.65 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Cellular tumor antigen p53 Chain: B
Molecule details ›
Chain: B
Length: 227 amino acids
Theoretical weight: 25.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 92-312; Coverage: 56%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 46.36Å b: 68.28Å c: 109.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.164 0.205
Expression system: Escherichia coli