PDBe 4pt6

X-ray diffraction
2.1Å resolution

The discobody: an engineered discoidin domain from factor VIII that binds v 3 integrin with antibody-like affinities

Source organism: Homo sapiens
Entry authors: Kym G, Shi K, Kamajaya A, Hamdouche S, Kostecki JS, Wannier T, Li B, Nikolovski P, Mayo SL

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Factor VIIIa light chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 19.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P00451 (Residues: 2190-2215, 2220-2220, 2222-2351; Coverage: 7%)
Gene names: F8, F8C
Sequence domains: F5/8 type C domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P1
Unit cell:
a: 32.449Å b: 38.446Å c: 48.842Å
α: 98.56° β: 91.77° γ: 111.03°
R R work R free
0.202 0.199 0.252
Expression system: Escherichia coli