› 4poo

X-ray diffraction
2.2Å resolution

The crystal structure of Bacillus subtilis YtqB in complex with SAM

Released:
DOI: 10.2210/pdb4poo/pdb
PDB entry 4poo coloured by chain, front view.
PDB entry 4poo coloured by chain, side view.
PDB entry 4poo coloured by chain, top view.
Source organism: Bacillus subtilis subsp. spizizenii str. W23
Primary publication:
Structural analysis of a putative SAM-dependent methyltransferase, YtqB, from Bacillus subtilis.
Park SC, Song WS, Yoon SI
Biochem Biophys Res Commun 446 921-6 (2014)
PMID: 24637210

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122699 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative RNA methylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 21.79 KDa
Source organism: Bacillus subtilis subsp. spizizenii str. W23
Expression system: Escherichia coli
UniProt:
  • Canonical: E0TY72 (Residues: 1-194; Coverage: 100%)
Gene names: BSUW23_14785, ytqB
Sequence domains: Putative rRNA methylase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand SAM 2 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 47.066Å b: 77.661Å c: 100.481Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.26
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Identification of a novel 5-aminomethyl-2-thiouridine methyltransferase in tRNA modification.
Cho et al. (2023)