PDBe 4pj8

X-ray diffraction
3.3Å resolution

Structure of human MR1-5-OP-RU in complex with human MAIT TRBV20 TCR

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Major histocompatibility complex class I-related gene protein Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 31.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q95460 (Residues: 23-292; Coverage: 85%)
  • Best match: Q95460-5 (Residues: 23-202)
Gene name: MR1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
TCR-alpha Chain: C
Molecule details ›
Chain: C
Length: 203 amino acids
Theoretical weight: 22.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
TCR-beta Chain: D
Molecule details ›
Chain: D
Length: 247 amino acids
Theoretical weight: 27.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P3121
Unit cell:
a: 113.57Å b: 113.57Å c: 227.981Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.163 0.213
Expression system: Escherichia coli BL21(DE3)