PDBe 4pj1

X-ray diffraction
3.15Å resolution

Crystal structure of the human mitochondrial chaperonin symmetrical 'football' complex

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
10 kDa heat shock protein, mitochondrial Chains: 1, 2, O, P, Q, R, S, T, U, V, W, X, Y, Z
Molecule details ›
Chains: 1, 2, O, P, Q, R, S, T, U, V, W, X, Y, Z
Length: 114 amino acids
Theoretical weight: 12.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T
UniProt:
  • Canonical: P61604 (Residues: 1-102; Coverage: 100%)
Gene name: HSPE1
Sequence domains: Chaperonin 10 Kd subunit
60 kDa heat shock protein, mitochondrial Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 558 amino acids
Theoretical weight: 59.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T
UniProt:
  • Canonical: P10809 (Residues: 27-556; Coverage: 93%)
Gene names: HSP60, HSPD1
Sequence domains: TCP-1/cpn60 chaperonin family

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41212
Unit cell:
a: 199.1Å b: 199.1Å c: 627.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.241 0.27
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T