PDBe 4piv

X-ray diffraction
2.3Å resolution

Human Fatty Acid Synthase Psi/KR Tri-Domain with NADPH and GSK2194069


Function and Biology Details

Reactions catalysed:
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+)
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate
An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Fatty acid synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 660 amino acids
Theoretical weight: 71.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: P49327 (Residues: 1109-2114; Coverage: 26%)
Gene names: FAS, FASN
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

Cofactor: Ligand NDP 2 x NDP
3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 48.221Å b: 85.761Å c: 85.995Å
α: 65.49° β: 89.96° γ: 87.22°
R R work R free
0.216 0.214 0.246
Expression system: Spodoptera frugiperda