› 4p4s

X-ray diffraction
3.3Å resolution

GMPPCP-bound stalkless-MxA

Released:
DOI: 10.2210/pdb4p4s/pdb
PDB entry 4p4s coloured by chain, front view.
PDB entry 4p4s coloured by chain, side view.
PDB entry 4p4s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Transient dimerization of human MxA promotes GTP hydrolysis, resulting in a mechanical power stroke.
Rennie ML, McKelvie SA, Bulloch EM, Kingston RL
Structure 22 1433-45 (2014)
PMID: 25295396

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149010 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Interferon-induced GTP-binding protein Mx1, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 32.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20591 (Residues: 70-342; Coverage: 41%)
Gene name: MX1
Sequence domains: Dynamin family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Interferon-induced GTP-binding protein Mx1, N-terminally processed Chain: B
Molecule details ›
Chain: B
Length: 346 amino acids
Theoretical weight: 38.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20591 (Residues: 43-662; Coverage: 52%)
Gene name: MX1
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GCP 2 x GCP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 60.29Å b: 66.76Å c: 170.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.231 0.305
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Membrane fission by dynamin: what we know and what we need to know.
Antonny et al. (2016)
12 more

4 mentions without citation

Effects of allelic variations in the human myxovirus resistance protein A on its antiviral activity.
Graf et al. (2018)
3 more