PDBe 4p4h

X-ray diffraction
3.4Å resolution

Caught-in-action signaling complex of RIG-I 2CARD domain and MAVS CARD domain

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Probable ATP-dependent RNA helicase DDX58 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 205 amino acids
Theoretical weight: 24.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95786 (Residues: 1-201; Coverage: 22%)
Gene name: DDX58
Sequence domains: Caspase recruitment domain
Structure domains: Death Domain, Fas
Mitochondrial antiviral-signaling protein Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 108 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7Z434 (Residues: 1-99; Coverage: 18%)
Gene names: IPS1, KIAA1271, MAVS, VISA
Sequence domains: Caspase recruitment domain
Structure domains: Death Domain, Fas
Ubiquitin Chains: S, T, U, W, X
Molecule details ›
Chains: S, T, U, W, X
Length: 79 amino acids
Theoretical weight: 8.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62987 (Residues: 1-76; Coverage: 59%)
Gene names: UBA52, UBCEP2
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 111.81Å b: 117.33Å c: 257.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.24
Expression system: Escherichia coli BL21(DE3)