PDBe 4p3d

X-ray diffraction
1.95Å resolution

MT1-MMP:Fab complex (Form II)

Released:

Function and Biology Details

Reaction catalysed:
Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Heavy Chain Fab fragment of antibody LEM-2/15 Chains: A, H
Molecule details ›
Chains: A, H
Length: 218 amino acids
Theoretical weight: 23.49 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Light Chain Fab fragment of antibody LEM-2/15 Chains: B, L
Molecule details ›
Chains: B, L
Length: 218 amino acids
Theoretical weight: 23.96 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Matrix metalloproteinase-14 Chains: C, M
Molecule details ›
Chains: C, M
Length: 13 amino acids
Theoretical weight: 1.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P50281 (Residues: 215-227; Coverage: 2%)
Gene name: MMP14

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 52.898Å b: 96.226Å c: 81.521Å
α: 90° β: 92.36° γ: 90°
R-values:
R R work R free
0.161 0.158 0.21
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21