PDBe 4p0p

X-ray diffraction
2.8Å resolution

Crystal structure of Human Mus81-Eme1 in complex with 5'-flap DNA, and Mg2+

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
3 distinct DNA molecules
Macromolecules (5 distinct):
Crossover junction endonuclease MUS81 Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96NY9 (Residues: 246-551; Coverage: 56%)
Gene name: MUS81
Sequence domains: ERCC4 domain
Structure domains: DNA polymerase; domain 1
Crossover junction endonuclease EME1 Chain: B
Molecule details ›
Chain: B
Length: 393 amino acids
Theoretical weight: 43.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96AY2 (Residues: 178-570; Coverage: 69%)
Gene names: EME1, MMS4
Sequence domains: ERCC4 domain
Structure domains:
DNA GAATGTGTGTCTCAATC Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.22 KDa
DNA GGATTG Chain: F
Molecule details ›
Chain: F
Length: 6 nucleotides
Theoretical weight: 1.86 KDa
DNA TAACCAGACACACATT Chain: G
Molecule details ›
Chain: G
Length: 16 nucleotides
Theoretical weight: 4.84 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 85.023Å b: 226.499Å c: 52.457Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.243
Expression system: Escherichia coli