PDBe 4ozi

X-ray diffraction
3.2Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class II histocompatibility antigen, DQ alpha 1 chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 191 amino acids
Theoretical weight: 21.52 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P01909 (Residues: 24-206; Coverage: 79%)
Gene name: HLA-DQA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DQ beta 1 chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 213 amino acids
Theoretical weight: 24.36 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5Y7D3 (Residues: 33-224; Coverage: 84%)
Gene name: HLA-DQB1
Sequence domains:
Structure domains:
TRA@ protein Chains: E, G
Molecule details ›
Chains: E, G
Length: 207 amino acids
Theoretical weight: 22.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q2YD82 (Residues: 1-86, 87-194; Coverage: 81%)
Gene name: TRA@
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chains: F, H
Molecule details ›
Chains: F, H
Length: 244 amino acids
Theoretical weight: 27.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Alpha/beta-gliadin A-II Chains: I, J
Molecule details ›
Chains: I, J
Length: 13 amino acids
Theoretical weight: 1.46 KDa
Source organism: Triticum aestivum
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P04722 (Residues: 79-89; Coverage: 4%)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 233.027Å b: 142.239Å c: 101.09Å
α: 90° β: 109.79° γ: 90°
R-values:
R R work R free
0.214 0.212 0.243
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21