PDBe 4owr

X-ray diffraction
3.15Å resolution

Vesiculoviral matrix (M) protein occupies nucleic acid binding site at nucleoporin pair Rae1-Nup98

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
mRNA export factor Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 38.09 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P78406 (Residues: 31-368; Coverage: 92%)
Gene names: MRNP41, RAE1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Nuclear pore complex protein Nup98 Chain: B
Molecule details ›
Chain: B
Length: 59 amino acids
Theoretical weight: 6.51 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P52948 (Residues: 157-213; Coverage: 3%)
Gene names: ADAR2, NUP98
Structure domains: Arc Repressor Mutant, subunit A
Matrix protein Chain: C
Molecule details ›
Chain: C
Length: 188 amino acids
Theoretical weight: 21.49 KDa
Source organism: Vesicular stomatitis Indiana virus (strain 85CLB South America)
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q8B0H7 (Residues: 43-229; Coverage: 82%)
  • Best match: Q8B0H7-3 (Residues: 1-179)
Gene name: M
Structure domains: VSV matrix protein

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P4212
Unit cell:
a: 141.372Å b: 141.372Å c: 78.462Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.213 0.289
Expression system: Trichoplusia ni