PDBe 4ouc

X-ray diffraction
1.9Å resolution

Structure of human haspin in complex with histone H3 substrate

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase haspin Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TF76 (Residues: 465-798; Coverage: 42%)
Gene names: GSG2, HASPIN
Structure domains:
Histone H3.2 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q71DI3 (Residues: 2-13; Coverage: 9%)
Gene names: H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 50.54Å b: 79.13Å c: 100.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.211
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided