PDBe 4oga

X-ray diffraction
3.5Å resolution

Insulin in complex with Site 1 of the human insulin receptor

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
monoclonal antibody fab 83-7 fragment - heavy chain Chain: C
Molecule details ›
Chain: C
Length: 118 amino acids
Theoretical weight: 12.89 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
monoclonal antibody fab 83-7 fragment - light chain Chain: D
Molecule details ›
Chain: D
Length: 114 amino acids
Theoretical weight: 12.68 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Insulin receptor subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 317 amino acids
Theoretical weight: 36.23 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06213 (Residues: 28-337; Coverage: 23%)
Gene name: INSR
Sequence domains: Receptor L domain
Structure domains: Hormone Receptor, Insulin-like Growth Factor Receptor 1; Chain A, domain 2
Insulin receptor subunit alpha Chain: F
Molecule details ›
Chain: F
Length: 16 amino acids
Theoretical weight: 1.92 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P06213 (Residues: 731-746; Coverage: 1%)
  • Best match: P06213-2 (Residues: 731-746)
Gene name: INSR

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P23
Unit cell:
a: 169.04Å b: 169.04Å c: 169.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.264 0.284
Expression systems:
  • Saccharomyces cerevisiae
  • Mus musculus
  • Cricetulus griseus
  • Not provided