PDBe 4oaa

X-ray diffraction
3.5Å resolution

Crystal structure of E. coli lactose permease G46W,G262W bound to sugar

Released:
Primary publication:
Structure of sugar-bound LacY.
Proc. Natl. Acad. Sci. U.S.A. 111 1784-8 (2014)
PMID: 24453216

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose/galactose transporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 46.79 KDa
Source organism: Escherichia coli str. K-12 substr. DH10B
Expression system: Escherichia coli
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 101.56Å b: 121.94Å c: 264.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.256 0.293
Expression system: Escherichia coli