› 4o3m

X-ray diffraction
2.3Å resolution

Ternary complex of Bloom's syndrome helicase

Released:
DOI: 10.2210/pdb4o3m/pdb
PDB entry 4o3m coloured by chain, front view.
PDB entry 4o3m coloured by chain, side view.
PDB entry 4o3m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human Bloom's syndrome helicase in complex with ADP and duplex DNA.
Swan MK, Legris V, Tanner A, Reaper PM, Vial S, Bordas R, Pollard JR, Charlton PA, Golec JM, Bertrand JA
Acta Crystallogr D Biol Crystallogr 70 1465-75 (2014)
PMID: 24816114

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117893 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
RecQ-like DNA helicase BLM Chain: A
Molecule details ›
Chain: A
Length: 659 amino acids
Theoretical weight: 75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P54132 (Residues: 640-1298; Coverage: 47%)
Gene names: BLM, RECQ2, RECQL3
Sequence domains:
Structure domains:
5'-D(*AP*GP*CP*GP*TP*CP*GP*AP*GP*AP*TP*CP*CP*AP*AP*G)-3' Chain: P
Molecule details ›
Chain: P
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*CP*TP*TP*GP*GP*AP*TP*CP*TP*CP*GP*AP*CP*GP*CP*TP*CP*TP*CP*CP*CP*TP*TP*A)-3' Chain: T
Molecule details ›
Chain: T
Length: 24 nucleotides
Theoretical weight: 7.25 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand ZN 1 x ZN
Ligand ADP 1 x ADP
Ligand EDO 8 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 100.93Å b: 164.68Å c: 50.98Å
α: 90° β: 90.14° γ: 90°
R-values:
R R work R free
0.183 0.181 0.215
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Bloom's Syndrome: Clinical Spectrum, Molecular Pathogenesis, and Cancer Predisposition.
Cunniff et al. (2017)
3 more

17 mentions without citation

Structural mechanisms of human RecQ helicases WRN and BLM.
Kitano K. (2014)
16 more