PDBe 4o27

X-ray diffraction
3.18Å resolution

Crystal structure of MST3-MO25 complex with WIF motif

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Calcium-binding protein 39 Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 38.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y376 (Residues: 11-333; Coverage: 95%)
Gene names: CAB39, CGI-66, MO25
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein kinase 24 36 kDa subunit Chain: B
Molecule details ›
Chain: B
Length: 281 amino acids
Theoretical weight: 31.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y6E0 (Residues: 30-309; Coverage: 63%)
Gene names: MST3, STK24, STK3
Sequence domains: Protein kinase domain
Structure domains:
5-mer peptide from serine/threonine-protein kinase 24 Chain: C
Molecule details ›
Chain: C
Length: 5 amino acids
Theoretical weight: 709 Da

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 119.111Å b: 119.111Å c: 149.258Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.196 0.26
Expression system: Escherichia coli