PDBe 4nri

X-ray diffraction
2.3Å resolution

Crystal Structure of a human Mms2/Ubc13 A122G mutant

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-conjugating enzyme E2 N Chain: B
Molecule details ›
Chain: B
Length: 156 amino acids
Theoretical weight: 17.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61088 (Residues: 3-150; Coverage: 97%)
Gene names: BLU, UBE2N
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
Ubiquitin-conjugating enzyme E2 variant 2 Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15819 (Residues: 6-145; Coverage: 97%)
Gene names: MMS2, UBE2V2, UEV2
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 43.612Å b: 78.187Å c: 92.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.235
Expression system: Escherichia coli BL21(DE3)