PDBe 4nm7

X-ray diffraction
2.3Å resolution

Crystal structure of GSK-3/Axin complex bound to phosphorylated Wnt receptor LRP6 e-motif

Released:

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
ATP + [tau protein] = ADP + [tau protein] phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Glycogen synthase kinase-3 beta Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49841 (Residues: 13-383; Coverage: 88%)
Gene name: GSK3B
Sequence domains: Protein kinase domain
Structure domains:
Axin-1 Chain: B
Molecule details ›
Chain: B
Length: 24 amino acids
Theoretical weight: 2.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15169 (Residues: 383-402; Coverage: 2%)
Gene names: AXIN, AXIN1
Low-density lipoprotein receptor-related protein 6 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75581 (Residues: 1602-1610; Coverage: 1%)
Gene name: LRP6

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P6122
Unit cell:
a: 82.002Å b: 82.002Å c: 280.345Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.234
Expression system: Escherichia coli