PDBe 4nm0

X-ray diffraction
2.5Å resolution

Crystal structure of peptide inhibitor-free GSK-3/Axin complex

Released:

Function and Biology Details

Reactions catalysed:
ATP + a protein = ADP + a phosphoprotein
ATP + [tau protein] = ADP + [tau protein] phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glycogen synthase kinase-3 beta Chain: A
Molecule details ›
Chain: A
Length: 389 amino acids
Theoretical weight: 44.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49841 (Residues: 1-383; Coverage: 91%)
Gene name: GSK3B
Sequence domains: Protein kinase domain
Structure domains:
Axin-1 Chain: B
Molecule details ›
Chain: B
Length: 24 amino acids
Theoretical weight: 2.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15169 (Residues: 383-402; Coverage: 2%)
Gene names: AXIN, AXIN1

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6122
Unit cell:
a: 81.303Å b: 81.303Å c: 280.829Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.24
Expression system: Escherichia coli