PDBe 4nkx

X-ray diffraction
2.79Å resolution

Human steroidogenic cytochrome P450 17A1 mutant A105L with substrate progesterone

Released:
Source organism: Homo sapiens

Function and Biology Details

Reactions catalysed:
A C(21)-steroid + [reduced NADPH--hemoprotein reductase] + O(2) = a 17-alpha-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H(2)O
17-alpha-hydroxypregnenolone + [reduced NADPH--hemoprotein reductase] + O(2) = 3-beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH--hemoprotein reductase] + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid 17-alpha-hydroxylase/17,20 lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 494 amino acids
Theoretical weight: 55.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P05093 (Residues: 24-508; Coverage: 96%)
Gene names: CYP17, CYP17A1, S17AH
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 85.934Å b: 153.036Å c: 173.243Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.184 0.257
Expression system: Escherichia coli