PDBe 4nkq

X-ray diffraction
3.3Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytokine receptor common subunit beta Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 47.26 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32927 (Residues: 25-438; Coverage: 47%)
Gene names: CSF2RB, IL3RB, IL5RB
Sequence domains:
Granulocyte-macrophage colony-stimulating factor Chain: C
Molecule details ›
Chain: C
Length: 127 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04141 (Residues: 18-144; Coverage: 100%)
Gene names: CSF2, GMCSF
Sequence domains: Granulocyte-macrophage colony-stimulating factor
Structure domains: Growth Hormone; Chain: A;
Granulocyte-macrophage colony-stimulating factor receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 305 amino acids
Theoretical weight: 34.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P15509 (Residues: 113-400; Coverage: 76%)
Gene names: CSF2R, CSF2RA, CSF2RY
Sequence domains: Interleukin-6 receptor alpha chain, binding
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P6322
Unit cell:
a: 166.656Å b: 166.656Å c: 213.132Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.229 0.273
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli