PDBe 4nh1

X-ray diffraction
3.3Å resolution

Crystal structure of a heterotetrameric CK2 holoenzyme complex carrying the Andante-mutation in CK2beta and consistent with proposed models of autoinhibition and trans-autophosphorylation

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Casein kinase II subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 215 amino acids
Theoretical weight: 24.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P67870 (Residues: 1-215; Coverage: 100%)
Gene names: CK2N, CSNK2B, G5A
Sequence domains: Casein kinase II regulatory subunit
Structure domains:
Casein kinase II subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 40.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P68400 (Residues: 1-335; Coverage: 86%)
Gene names: CK2A1, CSNK2A1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 210.537Å b: 57.441Å c: 139.394Å
α: 90° β: 118.54° γ: 90°
R-values:
R R work R free
0.215 0.213 0.251
Expression system: Escherichia coli