PDBe 4nge

X-ray diffraction
2.7Å resolution

Crystal Structure of Human Presequence Protease in Complex with Amyloid-beta (1-40)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Amyloid-beta protein 40 Chains: B, E
Molecule details ›
Chains: B, E
Length: 40 amino acids
Theoretical weight: 4.34 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05067 (Residues: 672-711; Coverage: 5%)
Gene names: A4, AD1, APP
Beta-amyloid protein 40 Chains: C, F
Molecule details ›
Chains: C, F
Length: 7 amino acids
Theoretical weight: 614 Da
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Presequence protease, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 1014 amino acids
Theoretical weight: 115.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5JRX3 (Residues: 33-1037; Coverage: 97%)
Gene names: KIAA1104, MP1, PITRM1
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain A, domain 1
Presequence protease, mitochondrial Chain: D
Molecule details ›
Chain: D
Length: 1014 amino acids
Theoretical weight: 115.57 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q5JRX3 (Residues: 33-1037; Coverage: 97%)
Gene names: KIAA1104, MP1, PITRM1
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain A, domain 1

Ligands and Environments

3 bound ligands:

3 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 247.275Å b: 86.183Å c: 158.611Å
α: 90° β: 127.56° γ: 90°
R-values:
R R work R free
0.193 0.191 0.232
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)