› 4n8v

X-ray diffraction
2.5Å resolution

Crystal structure of killer cell immunoglobulin-like receptor KIR2DS2 in complex with HLA-A

Released:
DOI: 10.2210/pdb4n8v/pdb
PDB entry 4n8v coloured by chain, front view.
PDB entry 4n8v coloured by chain, side view.
PDB entry 4n8v coloured by chain, top view.
Source organisms:
Primary publication:
Activating killer cell immunoglobulin-like receptor 2DS2 binds to HLA-A*11.
Liu J, Xiao Z, Ko HL, Shen M, Ren EC
Proc Natl Acad Sci U S A 111 2662-7 (2014)
PMID: 24550293

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-137698 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Killer cell immunoglobulin-like receptor 2DS2 Chains: G, I
Molecule details ›
Chains: G, I
Length: 201 amino acids
Theoretical weight: 22.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43631 (Residues: 22-221; Coverage: 71%)
Gene names: CD158J, KIR2DS2, NKAT5
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins
HLA class I histocompatibility antigen, A alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 274 amino acids
Theoretical weight: 31.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Virion membrane protein OPG140 Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.13 KDa
Source organism: Vaccinia virus WR
Expression system: Not provided
UniProt:
  • Canonical: Q76ZQ3 (Residues: 61-69; Coverage: 10%)
Gene names: A14L, OPG140

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 69.55Å b: 94.67Å c: 228.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.257
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

22 review citations

Killer Ig-Like Receptors (KIRs): Their Role in NK Cell Modulation and Developments Leading to Their Clinical Exploitation.
Pende et al. (2019)
21 more

5 mentions without citation

Activating killer cell immunoglobulin-like receptor 2DS2 binds to HLA-A*11.
Liu et al. (2014)
4 more