PDBe 4mzh

X-ray diffraction
2.2Å resolution

Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2s) peptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Spindlin-1 Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 25.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y657 (Residues: 50-262; Coverage: 81%)
Gene names: OCR, SPIN, SPIN1
Sequence domains: Spin/Ssty Family
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)
Histone H3.2 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q71DI3 (Residues: 2-10; Coverage: 7%)
Gene names: H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D

Ligands and Environments

1 bound ligand:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 122.985Å b: 41.315Å c: 50.461Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.196 0.247
Expression systems:
  • Escherichia coli
  • Not provided