PDBe 4mvk

X-ray diffraction
1.5Å resolution

Crystal structure of an engineered lipocalin (Anticalin US7) in complex with the Alzheimer amyloid peptide fragment VFFAED

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
P3(40) Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 751 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05067 (Residues: 689-694; Coverage: 1%)
Gene names: A4, AD1, APP
Neutrophil gelatinase-associated lipocalin Chain: A
Molecule details ›
Chain: A
Length: 188 amino acids
Theoretical weight: 21.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P80188 (Residues: 21-198; Coverage: 100%)
Gene names: HNL, LCN2, NGAL
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 50.97Å b: 59.23Å c: 61.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.186
Expression systems:
  • Not provided
  • Escherichia coli