PDBe 4msq

X-ray diffraction
1.95Å resolution

Crystal structure of Schizosaccharomyces pombe AMSH-like protease sst2 catalytic domain bound to ubiquitin


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AMSH-like protease sst2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 197 amino acids
Theoretical weight: 21.98 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21
  • Canonical: NEW Q9P371 (Residues: 245-435; Coverage: 44%)
Gene names: SPAC19B12.10, sst2
Sequence domains: JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Structure domains: Cytidine Deaminase, domain 2
Ubiquitin Chains: B, D
Molecule details ›
Chains: B, D
Length: 81 amino acids
Theoretical weight: 8.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: NEW P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 71.244Å b: 57.032Å c: 81.175Å
α: 90° β: 104.58° γ: 90°
R R work R free
0.178 0.177 0.207
Expression system: Escherichia coli BL21