PDBe 4mnq

X-ray diffraction
2.74Å resolution

TCR-peptide specificity overrides affinity enhancing TCR-MHC interactions

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01892 (Residues: 25-300; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Telomerase reverse transcriptase Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O14746 (Residues: 540-548; Coverage: 1%)
Gene names: EST2, TCS1, TERT, TRT
T-cell receptor, sp3.4 beta chain Chain: D
Molecule details ›
Chain: D
Length: 200 amino acids
Theoretical weight: 22.25 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: K7N5N2 (Residues: 115-203; Coverage: 43%)
  • Canonical: H2RG00 (Residues: 22-108; Coverage: 53%)
  • nullnull
Gene name: LOC452776
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chain: E
Molecule details ›
Chain: E
Length: 240 amino acids
Theoretical weight: 27.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 94.22Å b: 48.49Å c: 118.07Å
α: 90° β: 107.86° γ: 90°
R-values:
R R work R free
0.218 0.214 0.284
Expression systems:
  • Escherichia coli
  • Not provided