PDBe 4mne

X-ray diffraction
2.85Å resolution

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dual specificity mitogen-activated protein kinase kinase 1 Chains: A, D, E, H
Molecule details ›
Chains: A, D, E, H
Length: 341 amino acids
Theoretical weight: 37.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q02750 (Residues: 62-393; Coverage: 85%)
Gene names: MAP2K1, MEK1, PRKMK1
Sequence domains: Protein kinase domain
Structure domains:
Serine/threonine-protein kinase B-raf Chains: B, C, F, G
Molecule details ›
Chains: B, C, F, G
Length: 308 amino acids
Theoretical weight: 35.35 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: NEW P15056 (Residues: 432-726; Coverage: 39%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Protein tyrosine kinase
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 97.498Å b: 135.657Å c: 256.059Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.24
Expression systems:
  • Escherichia coli BL21(DE3)
  • Trichoplusia ni