PDBe 4mn4

X-ray diffraction
2.3Å resolution

Structural Basis for the MukB-topoisomerase IV Interaction

Released:

Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA topoisomerase 4 subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 259 amino acids
Theoretical weight: 28.3 KDa
Source organism: Escherichia coli str. K-12 substr. MG1655
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFI2 (Residues: 497-752; Coverage: 34%)
Gene names: JW2987, b3019, parC
Sequence domains: DNA gyrase C-terminal domain, beta-propeller
Chromosome partition protein MukB Chains: C, D
Molecule details ›
Chains: C, D
Length: 163 amino acids
Theoretical weight: 18.35 KDa
Source organism: Escherichia coli str. K-12 substr. MG1655
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22523 (Residues: 645-804; Coverage: 11%)
Gene names: JW0907, b0924, mukB

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 49.767Å b: 103.737Å c: 186.611Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.246
Expression system: Escherichia coli BL21(DE3)