PDBe 4mez

X-ray diffraction
2.05Å resolution

Crystal structure of M68L/M69T double mutant TEM-1

Source organism: Escherichia coli
Entry authors: Park J, Gobeil S, Pelletier JN, Berghuis AM

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Beta-lactamase TEM Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 28.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P62593 (Residues: 24-286; Coverage: 100%)
Gene names: bla, blaT-3, blaT-4, blaT-5, blaT-6
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 34.333Å b: 55.071Å c: 77.681Å
α: 70.83° β: 81.22° γ: 71.84°
R R work R free
0.195 0.192 0.248
Expression system: Escherichia coli