PDBe 4md7

X-ray diffraction
3.1Å resolution

Crystal Structure of full-length symmetric CK2 holoenzyme

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Casein kinase II subunit beta Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 215 amino acids
Theoretical weight: 24.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P67870 (Residues: 1-215; Coverage: 100%)
Gene names: CK2N, CSNK2B, G5A
Sequence domains: Casein kinase II regulatory subunit
Structure domains:
Casein kinase II subunit alpha Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 391 amino acids
Theoretical weight: 45.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P68400 (Residues: 1-391; Coverage: 100%)
Gene names: CK2A1, CSNK2A1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 142.316Å b: 57.958Å c: 186.188Å
α: 90° β: 102.42° γ: 90°
R-values:
R R work R free
0.235 0.233 0.263
Expression system: Escherichia coli BL21