4m72

X-ray diffraction
2.1Å resolution

Mutant structure of methyltransferase from Streptomyces hygroscopicus

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + 3-phenylpyruvate = S-adenosyl-L-homocysteine + (3S)-2-oxo-3-phenylbutanoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-179134 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylpyruvate C(3)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 337 amino acids
Theoretical weight: 37.21 KDa
Source organism: Streptomyces hygroscopicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q643C8 (Residues: 1-337; Coverage: 100%)
Gene name: mppJ
Sequence domains: Methyltransferase domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A
Spacegroup: P212121
Unit cell:
a: 60.871Å b: 97.136Å c: 135.234Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.144 0.196
Expression system: Escherichia coli